Theoretical uncertainty of measurements using quantitative polymerase chain reaction

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Title: Theoretical uncertainty of measurements using quantitative PCR Running Title: Quantitative PCR theoretical uncertainty

Current quantitative PCR protocols are only indicative of the relative quantity of a target sequence to a standard since no means of estimating the amplification rate is available as yet. The variability of PCR performed on isolated cells has already been reported by several authors, but it could not be extensively studied due to lack of a system for doing kinetic data acquisition and of statis...

Detection of Babesia ovis Using Polymerase Chain Reaction.

Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction.

We have developed a quantitative method for methylation analysis of the p16 gene based on real-time methylation-specific PCR (MSP). Real-time MSP is sensitive enough to detect down to 10 genome equivalents of the methylated p16 sequence. Application of real-time MSP to DNA from tumor-derived cell lines revealed complete concordance with conventional MSP analysis. Quantitative data generated by ...

Evolution of polymerase chain reaction to a quantitative laboratory tool.